Masterlist of Shoujo-ai

This is not of my creation, this belongs to Cuddlyness of shoujoai.com. I know shoujo-ai is rare to find so this is a good list to get shoujo-ai fans started. For those who don't know what shoujo-ai is, it's girl-love which means girl is attracted to girl. It's similar to shounen-ai (yaoi) however not so much emphasis on the physical. Don't flame this or I'll come torch your house =]

1) If a series name is marked with an (X) it means I've seen it, or as much of it as is currently available. Any commentary on these series is my own personal opinion and should not necessarily be taken as the consensus of the shoujo ai community, or any other "official" opinion. Feel free to offer your own opinions.
2) If a series is NOT marked with an (X), any comments about it are just what I've heard, mostly on these boards, in fact. Again, feel free to support or contradict what I've put, I'd be happy to learn the truth.
3) If a series name has nothing next to it, or at least nothing about the shoujo ai content, it means I know nothing whatever about it or its shoujo ai. I've merely heard it said that it has shoujo ai. Please expand on these series if possible.
4) If a series commentary contains the word "SAD", it means just that: it will break your heart. Avoid these if you can't bear unhappy endings.
5) If a series has an asterisk (*) before it's name, it is, IMHO, one of the best and/or most influential shoujo ai series. That is, it's one that I enjoyed and thought the shoujo ai was good, AND/OR, it is well-known in the shoujo ai community, AND/OR, it was an influential series in shoujo ai anime. Look to my comments for clarification. Keep in mind there may be many undiscovered gems, both in and out of this list, and that my opinion in no way makes or breaks a series.
6) If a series has two asterisks (**), it means that, IMHO, it is a transcendent, shining paragon of shoujo ai. It means the relationship is completely and utterly canon, and it means the relationship makes up a large part of the story itself. It means there are moments of beautiful and tender mutual love, and it means a happy ending, at least as it pertains to the relationship. Unfortunately, this will be a rare accolade.
7) Obviously, there will be spoilers present. Although I try to omit very specific details from the list, you are going to find out things about the series, possibly including the names of some characters, their shoujo ai pairings, the general way it ends, etc. Also, the messages below the list may contain any number of spoilers for any number of series. Read at your own discretion.

And now, the list:

.hack: //SIGN has canon shoujo ai, I've heard both good and bad things about it. I don't know anything about the other parts.
2x2=Shinobuden (X): Extremely hilarious fluff. My face aches from laughing so much. One of the most hilarious things I've ever seen, in anime or out of it. As for shoujo ai....Shinobu is very attached to Kaede, and definitely in a more-than-friends way. Her fantasies and behavior regarding Kaede leave little doubt. However, while Kaede is fond of Shinobu, there are no indications that she thinks of her as anything more than a friend, and anyway, Shinobu's yuri thoughts and actions are inevitably tied into comedy, as is the entire show. It's there if you want it, but it takes some suspension of disbelief. Apparantly, some people got a lot more out of it than I did. Draw your own conclusions.
A Little Snow Fairy, Sugar (X): An extremely well-executed and artistically beautiful series (both visually and aurally), Sugar is all about sweetness and cuteness, but never quite to excess. Depending on the amount of romantic connotation you want to apply to Saga and Sugar's powerfully emotional relationship, it can range from anything between "noticable subtext" to "near canon". However, the ending (and the Summer Special which an extended flashback) destroys any chance of them being together by forcing them apart forever after. It's not quite ruinously SAD, but it is very, very sad. On the other hand, we also have Saga/Greta. There can be little doubt that Greta is slightly obsessed with Saga, and is eager to prove herself to Saga and get noticed by her as much as possible. She also goes out of her way late in the series to try and do something very special for Saga. Subtext with potential. Finally, an honorable mention goes to Norma, who crushes on a pretty boy actor; a crush that, if anything, only intensifies when it's discovered "he" is actually a woman.
Agent Aika (X): Subtle hints between Aika and Rion, canon lesbian couple in later episodes (villains, but very devoted to each other). Also later in the series, a "bad guy" crossdressing woman attempts to seduce Aika and almost succeeds. The entire series is laden with panty fanservice, but it's so over the top it's more amusing than offputting. Quality fluff.
Air Master (X): Cute, a bit more serious than fluff, but not much more. Great series overall.
Alien Nine: Ambiguous/undeveloped. Less so in the manga, but still without much substance.
Angel/Dust (X): A very short, one-volume manga by Aoi Nanase. The relationship of Yuina and Seraph is very suggestive; there's a kiss (albeit a contrived one) at the beginning and their relationship is filled with vibes and mutual affection. Unfortunately, it's so brief that things happen very abruptly, and one could wish for more character development (I certainly wanted more). Still, what's there is enjoyable, and the ending, while ambiguous, is hopeful. One good thing about its shortness is you'll only have to spend about $10. Recommended.
Animation Runner Kuromi 1&2 (X): Two short but lovingly executed episodes of anime about making anime, this is a funny, interesting and entertaining look at a tiny animation house and the trials they endure just to meet deadline. There's some wonderful and charming interaction between the energetic Kuromi and the beautiful, quietly elegant director Shihon, especially in part 2. Subtext but very very enjoyable, and a very fun series overall.
Azumanga Daioh (X): Incredibly popular and deservedly so, this series seemlessly mingles comedy and weirdness with coming-of-age and friendship, all bound together in the endearing, unforgettable cast of characters. A true classic of anime. Regarding shoujo ai, secondary character Kaorin has a powerful crush on tall, quiet Sakaki. It's completely one-sided, and Kaorin's screen time is limited, but there's no denying the vehemence of her attraction. As for the rest of the all-female cast, almost none of them express the slightest romantic interest in anyone else, male or female, leaving room for a wide variety of pairings and speculation. If you love anime, see this.
Bakuretsu Tenshi/Burst Angel: I've heard varying opinions about this. Some say it's merely subtext that never develops, but I've also been told that the subtext is very strong and noticable, to the point of near-canon. I may need to see this one myself.
*Battle Athletes Victory (X): One of the best there is. Lighthearted, with very attractive animation and a large (almost all female) cast of interesting characters. Akari is such a sweetie, and she shows real character growth as the series goes on. The series is split into three main arcs, and for each the shoujo ai dynamic is different. In the first part of the series, there is a very strong, yet subtle, Akari/Itchan subtext. They make a great pair. However, in the second part of the story, Itchan is absent, and is replaced by the gloriously UNsubtle Kris Christopher. From the moment she sees Akari, she falls for her deeply, and pursues her in an extremely overt and energetic way. At first it's comedic and one-sided, but as things get more serious towards the end, they really draw together and come to mean a great deal to each other. They even share a wide-shot kiss, although it gets a bit blurred into Kris' spirituality. The final arc (which is sadly much shorter than the previous two) features both Kris and Itchan, bickering with each other over Akari and both of them displaying very clearly their affection for her (although Kris is still the much more blatant of the two). It ends happily for everybody, although in the future I can foresee a titanic battle between Kris and Itchan with Akari as the prize. ^_^
*Battle Athletes OVA (X): The six-episode OVA whose success gave birth to the Battle Athletes Victory TV series, this is a powerful and wonderful show in many ways superior to Victory (see above) IMHO. It is an alternate version of the same storyline and characters, but much more focused and serious, and the final episode contains some brief but potent and definitely canon Kris/Akari interaction, including a (mostly off-camera) kiss. However, it ends on a bittersweet if open-ended note. Nevertheless, I highly, highly recommend this.
Bleach: Apparantly has one openly lesbian character, Chizuru (no relation to Hen's Chizuru), and is rumored to have another, canon shoujo ai relationship later on.
Bubblegum Crisis 2033: Some say canon, some say subtext. Apparantly resolved, but SAD, I've been told.
Cardcaptor Sakura: Completely one-sided and unrequited, but popular nevertheless.
**Chirality (X): A gift from the Goddess of Shoujo Ai. Or rather, from the inimitable Satoshi Urushihara. This manga deserves to be counted among the highest tier of shoujo ai treasures. The breathtaking love that blossoms between Carol, a biological android created to save the world, and Shiori, the human girl whose gentle heart gave Carol a reason to trust humanity again, is beautifully and wonderfully portrayed. There are many, many sweet moments (and many kisses). There is yuri near the end, and for once, in the universe of anime/manga, it doesn't feel forced or out of place. It really feels born of a mutual love and desire to bond intimately with the other. Did I mention it all ends very happily? Do whatever it takes to read this.
Cinderella Collection: A manga by Yasue Imai, appears to have significant subtext between main character Nina and her friend Hiroka, but whether it goes anywhere, and how it ends, is unknown (by me, anyway).
Cosplay Complex (X): Fanservice and Fluff, but entertaining.
Cyberteam in Akihabara (X): One comedic but canon lesbian character in Episode 8, a few hints here and there between the main cast, but ultimately, there's really not much here, in my opinion. Otherwise, this is a fairly unremarkable, but still decently good sci-fi + magical girl show.
Detatoko Princess (X): An entertaining but brief fantasy anime parody wherein the "villainess" is trying to add the main character, Princess Lapis, to her "collection of pretty girls". It doesn't really go anywhere, but this was pretty cute overall. If you like Slayers, you'd probably like this (similar animation as well).
Devil Lady: Canon and reprocated, but SAD. I've been told it's still worth it though.
Dirty Pair Flash (X): In the second subseries, specifically the episode "Sparkle Bright Pure Love Flower Shop", there is a canon lesbian character in the very sweet flower girl. This particular episode begins with the Lovely Angel's male collegue crushing on this girl, and believing it's reciprocated, but at the end it's revealed that she is actually deeply in love with (the very appropriately named) Yuri. Yuri even apparantly agrees to go on a date with her, though we don't actually get to see this. It's a brief but pleasant surprise. Of course, there's always the Yuri/Kei question....I don't see it myself, but the debate is seemingly neverending.
Eatman 98: The OVA has some between side characters, but it's SAD. There's also a TV series and manga, but I don't know about them.
El-hazard: This is complicated. In "The Wanderers", there is some subtext between multiple characters. In "The Magnificent/Alternative World", there is as well, though what I've been told makes it sound more like fluff. Draw your own conclusions.
Erementar Gerald: Conflicting viewpoints, I've heard that there might be Rasati/Rerea subtext.
Excel Saga: Fluff with insanity.
Figure 17: SAD. Extremely SAD. I haven't seen this, and I don't plan to. If you want to, I won't spoil it for you, but the ending is just about as bad as it gets. Apart from that, I've heard it's pseudo-canon, but the participants are only 9 years old.
Gakuen Alice: Canon on one side, possibly reciprocated on the other is what I've been told.
Galaxy Fraulein Yuna (X): Interesting series. The main character, Yuna, has an enormous crush on a popular female superhero (whose alter ego is a friend of hers, but she doesn't know it) and also fantasizes about another girl quite vividly. There are tons of female characters here and no male ones at all. Lots and lots of subtext, but it's nothing serious. However, in the second half of the series, there is some very strong subtext, but it ends very SADly, to say the least...though painful there, most of the series is cute and humorous.
Ghost in the Shell: Motoko Kusanagi of Ghost in the Shell is bisexual. Apparantly though, it's inconsistently portrayed. Manga yes, movies no, and TV (Stand Alone Complex), well...I've been told it's pretty obvious, but also downplayed and not a big part of the show.
Girls Bravo, Season 2: This is a fanservice show by all accounts, but I've been told there is some fairly canon and cute shoujo ai between Kirie and Kosame. I sort of want to see this one myself. >.>
Gokujou Seitokai (X): An utterly fluffy and light series, this show is about the doings of an all-girl "maximum authority holders" student council. Basically, they run the school with a humorous pseudo-government-agency flair, and they conduct such missions as spying on their dating accountant and launching a student's pop idol career. There's a large, enjoyable cast of female characters and numerous bits of subtext among them. A fun and uncomplicated series.
Green Green: This seems to be a harem series, but I've been told Wakaba and Sanae are a yuri couple.
Haibane Renmei (X): Incredibly atmospheric and poignant storytelling, Haibane Renmei is one of the most excellent anime I've ever experienced. There is what could be called subtext between Rakka and Reki, but much like the show itself, their relationship is complex, multilayered, and open to interpretation. Whatever you choose to make of it, I exalt this series in the very highest of ways.
Hanaukyo Maid Tai La Verite (X): A underwhelming, formulaic maid/harem anime that nonetheless contains some canon but completely one-sided shoujo ai. It's humorous, fanservicy and utterly unrequited, but it is there. That being said, I don't recommend this series unless you have a strong stomach for maid/harem conventions.
Hayate X Blade: Not much is known yet, but this is being billed as a shoujo ai series, being based on work from the creator of fan-favorite Strawberry Shake. A "combat school" series apparantly, it involves the younger sister of a student masquerading as the latter. She becomes involved with her big sister's female sparring partner. More info as it develops.
Hen (First 16 chapters only): The manga is wonderful, from what I've seen and heard. It's about Chizuru, a very popular and beautiful, yet callous and cynical girl who falls completely and totally head over heels for Azumi, a petite, naive, innocent girl with a heart of gold. After struggling with denial, Chizuru submits to her feelings and goes to hilarious and elaborate lengths to be with and around Azumi, who is oblivious to the whole thing. There is some male/female sex which is not explicit, except for Chizuru's enormous breasts, which are prominently and frequently displayed throughout the manga. There is also the anime, known as "Strange Love" in the US, which is much shorter, unresolved, less about romance and more about ecchi situations. The manga ends happily, from what I've heard, but the scanlations are not completed, and may never be.
Iczer One: Canon and SAD.
Ikki Tousen (X): Nothing more and nothing less than a fanservice/action flick, heavy on the fanservice. There is a pairing here, Ryofu/Chinkyu, but they are both dead at the end, and weren't terribly engaging even before that. With that said, as fanservice shows go this isn't horrible, but don't watch it for the shoujo ai.
Jubei-chan 2 (X): Strong subtext at key points, semi-canon, but ultimately unresolved. Fairly happy ending, though.
Kagihime Monogatari (X): What begins as a very pedestrian magical girl/harem mixture from Kaishaku improves quite a bit after about the halfway point. There are a number of surprising plot twists, but in the end it's still rather lackluster. Kisa's feelings for Kiraha are absolutely canon, but completely one-sided.
Kaleido Star (Season One)(X): It would be impossible to express how fulfilled I was by this shoujo series. The main character, Sora, is fantastic, at once a shining example of humanity and a wonderfully normal everyday girl. The plot is relatively upbeat and excellent, the characters are enjoyable, the animation is gorgeous, the music is catchy. Frequently hilarious, often emotional, always meaningful. An example of anime at its best. However, the shoujo ai is only subtext. In the case of Sora/Layla, it seems to me rather to be a relationship of deep mutual trust, respect, and admiration, though it could be interpreted otherwise, mostly toward the end. There is also Sora/Rosetta, which strikes me as slightly more plausible, but unfortunately Rosetta only shows up very occasionally. Not the most substantial shoujo ai ever, but I can't recommend this series enough. It's pure gold.
Kaleido Star (Season Two): From what I've been told, the prominence of Layla has been reduced to make way for Rosetta, and there are some possibly suggestive vibes from them (just like the first season, in fact). I am absolutely going to see this at some point.
**Kannazuki no Miko (X): The Flagship Shoujo Ai Series. This series does more, and does it better, than any anime with shoujo ai ever has. It could not be more canon. It could not be more amazing, enthralling, fulfilling. The whole series is about the relationship of Chikane and Himeko (who is unaware of Chikane's true feelings for most of the series), and how Chikane, foreseeing the awful fate that awaits either her or Himeko, will do anything and everything necessary to save her beloved's life, no matter how painful it is or how much she must sacrifice. This series will tie your emotions in knots, but it will all be worth it in the end (just be sure to watch until the VERY end). The final episode is, in my opinion, the most beautiful, powerful, and romantic event in shoujo ai history, if not anime history altogether.
Kanon: Obvious subtext and reconciliation, but the characters are secondary.
*Kashimashi ~Girl Meets Girl~ (X): A cornucopia of shoujo ai, provided you accept the very true fact that Hazumu is a girl, and always was on the inside. Loved by both the shy, alienated Yasuna and the goldenhearted but emotionally-hamfisted Tomari, Hazumu feels she has to make a choice, but it's not quite that simple in the end...It's a funny, intriguing and heartwarming show with lots and lots of sweet girl-love. The ending (which is the 13th episode, not the 12th) is somewhat rushed and slightly unsatisfying because of it; nevertheless, it's a happy, canon ending and a wonderful series overall.
Key, The Metal Idol: Sad, one-sided feelings by Key for Sakura.
*Kiddy Grade (X): From beginning to end, this is a fantastic, gripping and well-constructed anime with great animation, great music, great story and great characters. It also has an admirable amount of shoujo ai. Eclair and Lumiere's relationship is tender and emotive. Lumiere's feelings for Eclair border on canon, and there are many gentle and meaningful moments between them. Eclair's feelings are not quite as clarified, but she cares deeply for Lumiere, and there's nothing to hinder its being or becoming quite mutual. Secondary character Tweedledee's interest in Lumiere, however, is not at all unclarified. Her time in the spotlight is short, but there can be little doubt, she is very intrigued. Shoujo ai fans should absolutely see this.
Knights of Ramune (X): Fanservice x 10 billion, but it's there...two couplings in fact. Not sure it's worth it though...
Kokoro Library (X): Very charming series. Pseudo-incestual love for younger sister by older sister, played largely for comedy.
Kurau: Phantom Memory: Subtext here, but I've been told it ends in a disappointing way.
Lemon Angel Project (currently airing): A music industry anime by Avex Mode in the tradition of Chance Triangle Session. A large female cast and, it would seem, some strong feelings between the lead character and her missing-in-action sempai. More info as it develops.
Love Hina: Hints in the anime, strong presence in the manga, but neither develops as a shoujo ai fan would like.
Loveless: This is primarily a shounen-ai show, however, episodes 8-10 center around the lesbian romance of two girls, Yamato and Kouya. It's canon, blatant, and very meaningful from what I've heard, if unfortunately brief.
Madlax: Multiple possibilities and potential pairings, but ultimately unsatisfying and cobbled together at the end.
Magical Girl Lyrical Nanoha (X): What a fantastic series. The first few episodes are deceptively formulaic mahou shoujo fare, but don't let that fool you. This is an amazing, riveting and emotional roller-coaster that steadily sheds the magical girl formulas and becomes glorious. The ending of the main plot is somewhat unresolved, but actually, it's seems like a good opening for another season. As for the shoujo ai, well, it's complicated. There can be absolutely no doubt that Arf loves Fate fiercely, but whether it's a romantic love is ambiguous. Nanoha becomes the center of Fate's attention several times late in the series, and you might expect Arf to betray some jealousy (I sure expected it), but it never happened. As for Nanoha/Fate, there is definitely some potential for this, though again, their relationship could be interpreted in a non-romantic way. I can't say there is anything unquestionably canon, but there is plenty of subtext here. Awesome series overall.
Mahou Shoujo Lyrical Nanoha A's (X): The second season of Nanoha upholds the quality of the original. The series overall is as glorious as ever, but the shoujo ai took a major hit this time. Fate/Nanoha interaction is pleasant but limited, the series focusing mainly on the newcoming characters. Vita could be seen to have a suggestive affection for Hayate, but that's about it.
Mahoraba (X): A warm, witty and wonderful series about friendship, affection and togetherness. A cast of funny and colorful characters inhabit a world of lush, vibrant animation, upbeat music, and heartwarming themes. The shoujo ai centers around Tamami (a somewhat cynical, lonely girl) and her powerful, possessive affection for her childhood friend Kozue, who is a virtual avatar of Sweetness and Light itself. The canon here is undeniable; as the series progresses, she does her utmost to keep Kozue to herself, and in the final episode Tama-chan fiercely and unequivocally proclaims her love for Kozue (proclaims it, that is, to Shiratori, the male lead, her perceived rival) and a little later cries when she realizes the selfish side of her affection. Sound angsty? It isn't, really. Tamami is comforted, and learns some important things about how to love and how to be more at ease with herself, Kozue, and those around her. Some might say Shiratori (who is a good and kind boy) has the upper hand at the end, but his interest in Kozue is soft-pedaled throughout the series, and Tama-chan is very obviously no less committed or forthright at the final curtain, even if she is less clingy. As for Kozue, she loves everybody and everything. The series is full of these themes; character-driven relationships and interaction, often poignant, but always happily resolved in the end, and lightened with excellent humor throughout. Well worth seeing.
Mai-Hime (X): A very entertaining series with something to interest almost everyone. Humor, romance (of almost every variety), action, angst, betrayal, suspense, the list goes on. There are in fact three potential couples here. By far the most prominent is Shizuru/Natsuki (affectionately known as "ShizNat" by the fans). Shizuru's feelings are canon to the point of near-obsession, and because of this she goes a bit off the deep end late in the series. Natsuki still likes her anyway, though not romantically, at least yet. Possibility but that's all. The same could be said for Mai/Mikoto. Mikoto is very, very devoted to Mai, but she's a rather simple girl who doesn't fully understand the (romantic) nuances of affection, and Mai definitely likes guys, two of which are pursuing her. Subtext with potential. Finally, Yukino/Haruka. I personally think this is impossible, but it's a popular and cute coupling. It has a SUPER happy ending for everybody, which actually doesn't seem to fit, according to a number of fans, but it's forgivable since it's so nice. All in all, this series is well worth watching, though I would not call it a paragon of shoujo ai.
Mai-Otome (X): Though not a direct sequel, this is, in every way, a continuation of Mai-Hime's formula. Hilarity, entertaining characters, incredibly overwrought hyperdrama and a fair sprinkling of action. Otherwise known as ShizNat Part II. While there's nothing all that overt here, they are undeniably together and have quite a few enjoyable moments together. Shizuru is awesome in any case. Ditto Chie/Aoi, though their screentime together is minor, and there are some small hints elsewhere, though they don't really develop. Watch Mai-Hime first, and if you want more with the great cast of characters and a ton of references and in-jokes, here it is.
*Maria-sama ga Miteru (X): When asked, many people might tell you that "Marimite" is the quintessential shoujo ai anime, despite the fact that with a sole exception, there's nothing canon here. An elegant shoujo series in the most classic yet flawless sense, it centers around the student council of an all-girl Catholic school, and their problems, interactions and relationships with each other. To say that the cast of characters are well-developed and memorable would be an understatement. Yumi, Sachiko, Sei, Shimako and the rest of the Yamayurikai and their aquaintences have become unbelievably popular icons of shoujo, shoujo ai, and anime in general. Sei is the only character to have had a canon lesbian relationship in her past; outside of that, everything is subtext, but the show is soaked with it. The dialog, the actions and reactions of the characters and their dynamics with the others all drip with suggestion. Almost any of the characters could be paired with almost anyone else, but there are some pairings with more weight than others. Yumi/Sachiko is the primary focus, but there is also a large Yumi/Sei following....there's a large Sei/Anyone following...Rei/Yoshino is considered pretty much a fact, as is Shimako/Noriko...look, there's just too much to go over here. If you're a shoujo ai fan, you should've already seen this. Get to it.
Maze (X): One part medieval fantasy action, one part bizarre-but-fascinating psychological drama, Maze the TV series is a glorious 90's anime that I greatly enjoyed. Princess Mill seems very much fixated on Female Maze and is not ambiguous about the nature of her feelings, but Maze's situation is highly complicated, and every viewer will have to decide for themselves. The show also contains a fantastic, positively depicted canon lesbian character in Solude, a smart, strong, self-confident and dependable woman. I found it wonderfully engrossing. There's also a two-episode, fanservice and humor-heavy OVA that could easily be skipped, but might still be fun for TV series fans.
Mermaid Melody Pichi Pichi Pitch: Reported as being extremely sugary and cute, a for-young-girls anime involving mermaids who battle each other with songs. Apparant subtext between "villains" SheShe and MiMi.
Miami Guns (X): A zany comedy "police girls" show with a fair bit of subtext throughout, but nothing overwhelming. Self-centered rich girl Yao and deadpan policewoman Lu are partners as they deal with exploding watermelons, giant octopi, and tofu delivery boys, among many other amusing situations. There are several hints that Lu is more than casually fond of Yao, and there is also a noticable Yao/Nagisa vibe. A semi-serious story arc does materialize toward the end, and it's fine and servicable in the tradition of police anime, though certainly nothing spectacular. A decent show with some shoujo ai potential, no more and no less. Some found the shoujo ai much more substantial than I did. Draw your own conclusions.
Miyuki-chan in Wonderland: Tons of lesbian fanservice courtesy of CLAMP. Fluffy and VERY short, but most people seem to have enjoyed it.
Neon Genesis Evangelion: This is EXTREMELY debatable, and a popular source of contention. Though I haven't seen the series, I always thought it was wishful thinking. However, I've been given to understand there are very, very subtle hints throughout, with something a tiny bit more concrete at the end of the Evangelion movie. SAD throughout, you'll have to make your own judgement on the level of shoujo ai here.
Najica Blitz Tactics: Apparantly canon, but I've heard conflicting takes on the ending, which is reportedly open to interpretation. Also there seems to be a villain(ess) involved. I've been told the manga is more satisfying from a shoujo ai perspective. Be aware that this show is the direct descendant of Agent Aika, and as such shares the same insane amount of panty and other fanservice, though it's mostly in good fun.
*Noir (X): Strong subtext between Mireille and Kirika, Chloe's feelings for Kirika are undeniably canon. Mireille/Kirika is considered canon by many. One of the most popular shoujo ai series.
One Piece: A popular shounen series. According to some, this has a near-canon lesbian character in Nami, who gets into suggestive relationships with girls named Vivi and (later) Robin; however, the degree of this is a matter of some contention among fans. It is a huge and ongoing series, and not one I intend to see myself, so prospective viewers are invited to judge for themselves.
Onii-sama E/Brother Dear Brother: Very twisted, very SAD.

 

Pitaten (X): Sorry, but I just don't see it. There's not even a hint before the last few episodes, and even then it just strikes me as the very emotional Misha being concerned for her friend.
Plastic Little (X): A gorgeous dose of action/fanservice by Urushihara, with a decently entertaining upbeat story, and some noticable subtext between Tita and Elysse. It doesn't resolve, but I would call it open-ended. Unfortunately it's short, only about an hour long.
Popotan (X): An odd and offbeat show with lots of fanservice, Popotan nevertheless manages to have quite a few meaningful moments, although by and large the essential mechanics of what's happening are never explained. Mai and Konami do share a suggestive bond, although their situations would seem to make it unlikely they could ever be together, the ambiguous ending of the show notwithstanding. Still, it's moderately strong subtext. This isn't for everyone, but it could be interesting if you have an open mind.
Pretty Cure: Cute with hints, but it seems debatable. More opinions, please.
Pretty Sammy: Subtext between Sammy and Misao. Close relationship with hints of something more.
Princess Tutu: Ambiguous.
Project A-ko: Canon feelings by B-Ko for C-Ko in the movie. Also in the OVA, plus some apparant A-Ko/C-Ko subtext. In the "Vs." OVA, there is still canon B-Ko for C-Ko, but this time there's also A-Ko/B-Ko subtext. I'm as confused as you are.
Puchi Puri Yuushi: Canon but unreconciled. Only appears later in the series. Reportedly is forgotten about as things move on.
Puni Puni Poemi (X): Charming, but fluff. Could've been much more but the insanity of this series makes it impossible to take seriously.
Ragnarok City (X): Another treat by the ever-inimitable Satoshi Urushihara, this is a "sexy visual story", basically a full-color manga. Not much actually happens apart from the completely female cast being displayed in various nude and might-as-well-be-nude outfits, but the relationship of Reicheiru and Kaede is obviously canon and sweet. Apart from the "story" plates there's also quite a bit of yuri artwork of the pair. This is fluff, and brief fluff at that, but it's charming nonetheless. Not the second coming of Chirality by any means, but anyone who loves shoujo ai/yuri and nude females in general should partake of this visual feast.
Ragnarok the Animation: In no way related to Ragnarok City, actually it is an anime based on the Ragnarok Online MMORPG. Apparantly it has some pseudo-canon shoujo ai, but it's brief and ends very, very SADly.
*Re: Cutey Honey (X): WONDERFUL. Exciting, action-packed, and with a very sweet, canon relationship between Honey and the prickly but warm-hearted Natsuko. A lot of cute moments, and some pretty moving scenes as well. This is sandwiched between mountains of fanservice, but it's done in a very comedic and tongue-in-cheek way, rather than being sleazy. Unfortunately, it's short...3 45-minute episodes, and you'll wish there was more. But what's there is great. Ends happily too.
*Revolutionary Girl Utena (X): This series is a mindbending fairy tale turned on its head. Memorable characters, bizarre music, lots of symbolism, a "villain" you will truly despise, and very strong subtext between Utena and Anthy. Considered canon by many, this is one of the most popular shoujo ai series, as well as a true anime classic. In my opinion, it is one of the greatest achievements of the medium. Goes hand in hand with the movie (see below).
*Revolutionary Girl Utena - Adolescence Mokushiroku (X): AKA the Utena Movie. Multiple kisses, it's considered proof of canon in the series (in the rare cases people need proof...) It's an EXTREMELY condensed alternate version of the series, and is almost too surreal and symbolic to take seriously (IMHO), but overall you are left with very little doubt as to the nature of the relationship of Anthy and Utena. Also it's some of the most gorgeous animation you are likely to see anywhere.
ROD OVA (X): Strong hints and subtext, but unresolved, semi-sad ending.
ROD TV (X): Strong hints and subtext for Yomiko/Nenene. Hisami's feelings are obviously canon, and Anita appears to reciprocate. Some bad moments near the end, but it turns out pretty well.
Rose of Versailles: SAD. (slight subtext)
*Sailor Moon (see comments): Slight subtext and multiple possibilities throughout, one canon lesbian couple in Haruka/Michiru. Considered a classic shoujo ai series and anime series in general, very popular and well-known. I watched the first season and some more a long time ago, before I really knew about shoujo ai.
Sensei no Ojikan: Apparantly, Kitagawa is very much absorbed with her childlike female teacher, but there seems to be some uncertainty as to whether it's actual attraction or merely a cuteness thing. Sounds one-sided, in any case.
Seraphim Call (X): The best analogy for this series is a short story compilation. There are 12 episodes in all and 11 girls. Each girl gets a single episode as their short story, with a final 12th episode wrapping things up. The 11 range from lighthearted fluff to thoughtful drama to mindbending surrealism. Shoujo ai is laced throughout several of the episodes, most notably 5, 6, and 12. Five and Six are devoted to a pair of twin girls, Shion and Sakura. Each is obsessed with the other, and with the aid of virtual reality devices they attempt to understand the psyche of the other, and their own. It becomes virtually impossible (kill me later) to tell their reality apart from their dream worlds, but there's a very nice kiss at the end of both their episodes, so you probably won't end up minding too much. The final episode is the best one IMHO, when all the girls finally meet in various, often entertaining ways. One of the girls, Kurumi, confesses love very forwardly and seriously to another, Chinami, who very amusingly asks Kurumi to take off her glasses, so Chinami can decide whether or not Kurumi looks pretty enough for her (and apparantly is satisfied with what she sees. n.n) Which, by the way, if you're a fan of megane-ko, girls with glasses, you will like Seraphim Call, as there are several of them. The animation, while looking a bit dated, is detailed and colorful, very nice overall. This show definitely won't be everybody's cup of tea, but if you like to experiment, it might be interesting to you.
Serial Experiments Lain: SAD.
Shiroi Heya no Futari: Manga. A very early work, possibly one of the earliest shoujo ai manga. A schoolgirl named Resine rooms with a "popular, beautiful and rebellious" girl named Simone, and the feelings they develop for each other. However, this was written during the dark ages of shoujo ai, so be prepared for SADness.
Silent Mobius 2: Strong subtext with an open ending.
Simoun: A new series still coming out, this show involves a planet where everyone is born female, then later chooses their permanant gender at 15 years of age. However, due to a war and the ancient weapons that can only be utilized by females, a group of young female (for the moment) soldiers has put off their choice in order to fight. There seems to be a lot of kissing here, but of course in the end the deciding factor will be who changes into what gender permanantly. One to keep an eye on.
Slayers (first season only): This is debatable, but personally, I find subtext between Lina and Sylphiel in the later portions of the first season. Each goes out of their way to help the other, and there are some endearing moments. One of my favorite "fanon" pairings. I've also heard there are hints between Lina/Naga in the movies, but that too appears arguable.
Sol Bianca - The Legacy: Lots of subtext between April and Feb(rurary?).
Sousei no Aquarion: An apparant comedy/mecha series. Extremely "cheesy" by all accounts, nevertheless there is apparant subtext between Tsugumi and Reuka. Mixed opinions on the overall quality of this series.
St. Luminous Mission High School (X): I didn't enjoy this series much. Nice kiss in the final episode though.
Steel Angel Kurumi 1&2 (X): Season 1=Cute, but fluff, unrequited. Season 2=Much more, still mostly fluff, still largely unresolved.
Stellvia of the Universe (X): This is a fine and solid sci-fi series with a sufficiently interesting story and likeable characters. Male lead Kouta and his "relationship" with Shima have all the chemistry and personality of a chunk of lead, but if you can get past that, there is much to like here. Arisa's tender attention to Shipon can hardly be interpreted any other way but loving affection. Forceful vibes from her throughout the series. Yayoi/Ayaka has limited screentime but their interaction is laden with meaning, and becomes virtually canon at the end. There's also a very charming secondary hetero couple, if you like such things. Presuming you can endure Kouta (AKA Generic Soulless Male Lead #39495848), this is a very worthwhile show.
*Strawberry Panic (X): An anime specifically about shoujo ai, nothing more and nothing less. Set in a complex of three all-girl schools, this series follows a large cast of young ladies as they get into romantic entanglements with each other, most notably the naive Nagisa, devoted Tamao, and the embittered but elegantly mischievous Shizuma. There are four main pairings plus numerous side hints among others, and in the end we get two major canon resolutions and one minor. While angsty and formulaic at times, this series also features some very endearing characters and beautiful moments, and plenty of shoujo ai goodness. Certainly one shoujo ai fans would find noteworthy.
Tales of Eternia: Slight hints in the final episodes.
Tenchi Muyo (OVA, Universe, in Tokyo): Throughout the various incarnations of Tenchi, there exists an embattled but persistent shoujo ai following, usually revolving around Ryoko/Ayeka and Kiyone/Mihoshi. Personally, I find the latter debatable and vigorously disagree with the former; nonetheless, these remain popular couplings in the fandom.
Utakata: Lots of subtext, but in the end, it sort of fizzles. They dance around it tauntingly, but the relationship of the two female leads makes it difficult or impossible for them to be permanantly together.
Vandread: Multiple possibilities "confirmed or implied". Can't speak for it myself, but as the women in the series apparantly come from an all-female culture, it would certainly make sense. I've heard this series contains a lot of "harem" anime conventions, and that the shoujo ai is mainly to be found in the second season. Some liked it, some apparantly didn't. I'm not all that interested in it personally, but you never know.
Variable Geo (X): Hints and subtext abound; there is at least one bi/lesbian female, though we don't see much of her. It's a fanservice flick, but a fairly good one IMHO.
Witchblade: Shiori and Aoi, the henchwomen of Reina and Maria (respectively) apparently have a thing for their mistresses.
Yamamoto Yohko TV (X): A quite mediocre sci-fi "high school girl space pilot show". In the next-to-last episode there is a single statement by Ayano that could be thought to be suggestive, but it is by no means worth watching this for. Faint subtext at most.
*Yami to Boushi to Hon no Tabibito (X): Very passionate, very powerful, very canon, very SAD. This series contains some very strong, mutual emotions between Hazuki and Hatsumi/Eve, but it ends in horrible disappointment. For better or worse, it has been very influential in the shoujo ai community.
Yokohama Kaidashi: Manga with a few OVA episodes. I've heard it's canon, but that's all I've heard. More info required.

Video Games

This is more difficult. A lot of video game characters don't get much personality, so it's easy to project shoujo ai onto situations where there are females who have some kind of interaction. Ovelia/Agrias in FFT and Ritz/Shara in FFTA are examples of this type of speculation. "Real" shoujo ai in video games is notoriously rare, which makes low-basis slashes more common as people mold the fill-in-the-blank characters to fulfill their shoujo ai yearning. Let's try to keep this list to only those games where there is just a bit more than potential.

Dark Stalkers 3: Morrigan/Lilith innuendo apparantly, though expect nothing substantial.
DOA Extreme Beach Volleyball 1&2: Though largely male-targetted fanservice games, there exists a shoujo ai following for this series. With a large, all-female cast of characters, there are many potential pairings, though how much of this is "fanon" is in the eye of the beholder.
Fallout 2: Lets you play a lesbian character. Apparantly this leads to some amusing situations. Not sure if anything substantial develops, though.
Final Fantasy X/X-2 (played both): Yuna/Rikku. THIS isn't wishful thinking? Maybe...there isn't a lot to support it, apart from the big hug scene in X (which did make my eyebrows go up). Then there's that whole Tidus deal. However, they are clearly very fond of and comfortable with each other. Also, in X-2 you are given a choice on how to resolve the Tidus question. There's subtext here, but it involves a bit of interpretation (i.e. wishful thinking. >_>;
Fatal Frame 2: "Well, if you don't have a problem with incest....our dear twin heroines have a lof of subtext going on...." Also, apparantly the Director's Cut has a happier, more shoujo ai friendly ending.
Fear Effect 1 & 2: "Confirmed lesbian main characters."
Fire Emblem: Rekka no Ken (X): Known simply as "Fire Emblem" in the US, we have Lyndis/Florina. Their "Support" conversations leave very little doubt in my mind. The third one is very strongly suggestive. They're also the only girl/girl pair to get a special ending, although...it says they parted, at least for that time. A bit inconsistent with their dialogue, which featured this tidbit: "Let's stick together, no matter what happens. Now...and always." There are numerous other girl/girl "Support" conversations, so there may be more shoujo ai here.
Jade Empire: A Chinese period-style game where you can play a female character and cultivate a romance with Princess Sun Lian, the Heavenly Lily. I've been told there's a fadeout kiss scene, and depending on how you play, a happy ending.
Knights of the Old Republic: I've heard some good things about this game. There's a female NPC, Juhani, who is a lesbian, and if your character is female, you can develop a relationship with her. I've been told it's subtle but undeniable.
Outlaw Golf 2: Has a pair of lovely lady announcers/cheerleaders who engage in a variety of suggestive activities. More fluff than substance though, it would seem.
Persona 2: Eternal Punishment: Subtext between secondary characters Noriko and Anna.
Rumble Roses: Apparantly, it's an all-girl wrestling game with lots of subtext between multiple girls.
Rumble Roses XX: Like its predecessor this is largely a fanservice game, Aigle and Makoto are said to have shoujo ai vibes.
SaGa Frontier (X): Asellus' path is riddled with it. Asellus/White Rose is fairly canon, as is Gina's feelings for Asellus, though that seems to be somewhat one-sided. Mesarthim the mermaid also seems very taken with Asellus. As a matter of fact, almost all female characters seem to be attracted to Asellus. There's a logical reason for that in her story, but it's a spoiler.
Sims 2: I add this under protest, since it's not really Japanese/anime-related, but there are lots of possibilities for lesbian dalliance. Don't expect emotional catharsis though.
Star Ocean: Till The End of Time (X): AKA Star Ocean 3. There is some very strong, multifaceted subtext between Nel and Clair. I consider it canon, myself.
Suikoden V (unfinished): A great game I haven't yet gotten around to finishing. One character, Lelei, is virtually confirmed to be a canon lesbian, and is very obviously smitten with her self-appointed "Lady", Lucretia. Lucretia herself, though a far more subtle character, seems to stealthily reciprocate Lelei's attentions. There's even a bit of dialogue that carries a veiled implication that they sleep together.
Summon Night: Swordcraft Story: Though I've not yet played it myself, this could be the first must-play shoujo ai game. A quirky GBA game, Summon Night allows you to play as Pratty, a female apprentice Craftknight. There are several female support characters who have varying levels of attraction to whichever character you picked, a fact that does not change if you happened to pick Pratty, who though somewhat embarassed by the attention, doesn't seem all that opposed to it either. ^_^ One of the girls, Sugar, is pretty much canon, but Sanary and Razzy also have claims to make. Though I can't vouch for the gameplay I've heard it's simplistic but fun, and the dialogue and characters are offbeat and endearing. Definitely of interest to shoujo ai-loving gamers.
Vampire the Masquerade - Bloodlines: As you might expect, this game is about vampires, and you play as one. Apparantly, there are a number of situations where you can become involved with female NPCs who don't seem to care whether or not your character is male or female.
Xenosaga Episode 1 (unfinished): There seems to be a fairly popular pairing in Shion/KOS-MOS. I didn't notice this during my abortive playthrough. Apparantly, there are hints in Episode 2 as well.

 

that guy is looopy